Dataset for the paper “Oujja, M., Agua, F., Sanz, M., Morales-Martín, D., García-Heras, M., Villegas M.A., Castillejo, M. 2021. Multiphoton Excitation Fluorescence Microscopy and Spectroscopic Multianalytical Approach for Characterization of Historical Glass Grisailles. Talanta 230, 122314” [Draft]

Laser-induced Breakdown Spectrocopy (LIBS) analyses of glass without grisaille (body surface) and the grisaille itself were performed using laser excitation at 266 nm (Nd:YAG laser, 6 ns pulses, 10 Hz repetition rate; Quantel Brilliant) and a 0.30 m spectrograph with a 1200 grooves/mm grating (TMc300 Bentham) coupled to an intensified charged coupled detector (ICCD, 2151 Andor Technologies). The fluence was of 6.6 J cm− 2. LIBS spectra were recorded with a gate delay and width of 100 ns and 3 μs, respectively. For the Laser-induced Fluorescence (LIF) analyses, the same equipment described above was used. However, time gate was operated with a zero-time delay, the grating was 300 lines/mm and the number of accumulations of 25. The fluence was 6 × 10− 3 J cm− 2. A home-made nonlinear optical microscope (NLOM) was used for Multi-Photon Excitation Fluorescence (MPEF) measurements of grisaille thickness with a mode-locked Ti:Sapphire femtosecond laser. The laser emits at 800 nm, with an average power of 680 mW, delivering 70 fs pulses at a repetition rate of 80 MHz and a laser power of 6–9 mW. The laser beam was modulated using a chopper at a frequency of 130 Hz and conducted to the sample through the aperture of a microscope objective lens (M Plan Apo HL 50X, Mitutoyo, NA 0.42). The focal plane of the laser was selected with motorized translation XYZ stages (Standa 8MVT100-25-1 for XY and Standa 8MTF for Z). In-depth cross section imaging of carbon coated glasses decorated with grisaille paint was carried out by Field Emission Scanning Electron Microscope (FESEM) with a Hitachi S-4800 cold cathode equipment, working with acceleration voltage of 15 kV. An Oxford X-Max of 20 mm2 system coupled to the electron microscope with resolution of 125 eV (Mg Kα) was used for Energy Dispersive X-Ray Spectroscopy (EDS) microanalyses. More details of the experimental conditions are available in their respective data folder. Data life: 2021- (unlimited validity). This dataset is subject to a Creative Commons Attribution 4.0 International (CC BY 4.0) License.

• Spanish
• English

• From 2021-06-01 02:00 to 2021-06-01 02:00

• http://hdl.handle.net/10261/244443
• https://www.wikidata.org/wiki/Q47290759
• https://www.wikidata.org/wiki/Q2261322
• https://www.wikidata.org/wiki/Q175874
• https://www.wikidata.org/wiki/Q175874
• https://www.wikidata.org/wiki/Q6058031

Recommended citation: Oujja, M., Agua Martínez, F., Sanz, M., Morales-Martín, D., García Heras, M., Villegas Broncano, M. Á., … Carmona-Quiroga, P. (2021). Dataset for the paper “Oujja, M., Agua, F., Sanz, M., Morales-Martín, D., García-Heras, M., Villegas M.A., Castillejo, M. 2021. Multiphoton Excitation Fluorescence Microscopy and Spectroscopic Multianalytical Approach for Characterization of Historical Glass Grisailles. Talanta 230, 122314” [Data set]. DIGITAL.CSIC. http://doi.org/10.20350/DIGITALCSIC/13919